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Draw a Lineweaver Burke plot for the xylose inhibition data compared with the uninhibited enzyme for the substrate you used (MANNOSE).

Generating graphs from given data with PRISM-GRAPHPAD

b) Analysis of the created graphs and results

c) Answering related questions

OVERALL:
(1) Draw a Direct Plot and a Lineweaver Burke plot from class data. Each graph will have three datasets (Fructose, Mannose and Glucose).

(2) Present a table giving the values obtained from your graphs for Km and Vmax for each substrate. You will need to convert the Vmax values to μmoles/min/1mL assay.

(3) Calculate the relative specificity constants using the Km and Vmax values.

(4) Draw a Lineweaver Burke plot for the xylose inhibition data compared with the uninhibited enzyme for the substrate you used (MANNOSE). Two lines as a minimum on this graph depending on your experimental design.

ANSWER THE FOLLOWING QUESTIONS:
1. Which is the best substrate? Why?
2. Which substrate has the lowest Km?
3. Is xylose an inhibitor? If so what is the type of inhibition?

What is quality control, quality management, SPC, Lean and 6- Sigma

History of company
2. History of M & M’s
3. Manufacturing process and production volume
4. Any historical Supply Chain issues or problem
5. Ten citations from 10 different sources

III. Role of Quality Control, Quality Management
1. What is quality control, quality management, SPC, Lean and 6- Sigma
2. How does this quality control impact sustainability
a. People
b. Planet
c. Profit
3. What tools are available to conduct an Spc analysis